http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H11506605-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 1996-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1999-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H11506605-A |
titleOfInvention | Method of masking probes to reduce background in amplification reactions |
abstract | (57) Abstract: A method for reducing the background caused by the target-independent production of an amplification product, typically the product of a ligase chain reaction or a polymerase chain reaction, wherein the amplification probe or primer is chemically Includes "masking" or blocking so that the probe or primer cannot be extended or ligated until a trigger event occurs that can delay until the start of the amplification reaction. The probe mask has the form of a complementary blocking oligonucleotide that cannot itself function as a template, preventing random tailing of the probe / primer. The blocking oligomask is denatured from the probe during amplification and is preferably effectively excluded from competition for the probe in the amplification reaction. |
priorityDate | 1995-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 107.