abstract |
PROBLEM TO BE SOLVED: To produce gamma-carboxylated, correctly folded and processed functional protein C in a eukaryotic host cell. SOLUTION: (a) DN encoding protein C A sequence is introduced into a recombinant vector, (b) The human fetal kidney cells transformed with the adenovirus are introduced, and the host cells of step (b) (c) are cultured under growth conditions in a medium that contains sufficient vitamin K for carboxylation. |