| abstract |
PROBLEM TO BE SOLVED: To obtain a high-purity soluble thrombomodulin substantially containing no serum-derived product or antibody-derived product. SOLUTION: An animal cell capable of producing soluble thrombomodulin is cultured using a medium containing a serum component, The obtained culture supernatant is brought into contact with an antibody against the soluble thrombomodulin, and then eluted. And obtaining a soluble thrombomodulin as a flow-through fraction by contacting the soluble thrombomodulin with a cation exchanger. |