| abstract |
PROBLEM TO BE SOLVED: To provide a monoclonal antibody suitable for monitoring PAH by immunoassay, a hybridoma cell line producing the antibody, and a PA in a sample using the antibody. An immunoassay for analyzing H is provided. Furthermore, the present invention provides a PAH conjugate useful as an immunogen in producing the antibody or as a standard in a competition assay. SOLUTION: The present inventors prepared a novel PAH conjugate by binding PAH to a carrier protein via a spacer, immunized an animal using the conjugate as an immunogen, and produced an antibody from the immunized animal. Hybridoma cell lines were prepared by fusing cells with myeloma cells, and by appropriately selecting these hybridoma cell lines, a hybridoma cell line producing an anti-PAH monoclonal antibody suitable for PAH measurement was established. Using this monoclonal antibody, an immunoassay for PAH measurement can be constructed. |