http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H11243996-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad2cd796f8c4dcb425ddcc68707d3444 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-185 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 1998-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7458ea7c7e1bf6ab597a42996661d9ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d89a3f927705132fe953bd0762654a5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_160992d5abe75af8fd138745077a279f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4620a8b316dfecd358ca173168600edf |
publicationDate | 1999-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H11243996-A |
titleOfInvention | Method for detecting insertion of foreign DNA fragment generated in verotoxin gene |
abstract | [PROBLEMS] To specifically detect a verotoxin gene or a mutant gene thereof of verotoxin-producing Escherichia coli, and at the same time, to cause an insertion mutation caused by insertion of a foreign DNA fragment into the gene. Is detected simply and quickly from the chain length of the amplified DNA fragment. [Solution] Vero toxin 1 produced by Vero toxin-producing Escherichia coli Gene encoding the type (SEQ ID NO: 1) or Vero toxin 2 Selected from the gene encoding the type (SEQ ID NO: 2), Oligonucleotides for amplifying at least two types of DNA fragments, vero toxin gene, containing a nucleic acid sequence consisting of at least 15 consecutive bases, method for amplifying vero toxin gene using said oligonucleotide as amplification primer, for amplification Exogenous D generated in reagent and verotoxin A method for detecting insertion of an NA fragment and a reagent for the detection. [Effect] Inactivation of the VT gene due to transfer of a foreign DNA fragment can be detected. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015146786-A |
priorityDate | 1998-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.