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filingDate 1998-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7458ea7c7e1bf6ab597a42996661d9ce
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publicationDate 1999-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H11243996-A
titleOfInvention Method for detecting insertion of foreign DNA fragment generated in verotoxin gene
abstract [PROBLEMS] To specifically detect a verotoxin gene or a mutant gene thereof of verotoxin-producing Escherichia coli, and at the same time, to cause an insertion mutation caused by insertion of a foreign DNA fragment into the gene. Is detected simply and quickly from the chain length of the amplified DNA fragment. [Solution] Vero toxin 1 produced by Vero toxin-producing Escherichia coli Gene encoding the type (SEQ ID NO: 1) or Vero toxin 2 Selected from the gene encoding the type (SEQ ID NO: 2), Oligonucleotides for amplifying at least two types of DNA fragments, vero toxin gene, containing a nucleic acid sequence consisting of at least 15 consecutive bases, method for amplifying vero toxin gene using said oligonucleotide as amplification primer, for amplification Exogenous D generated in reagent and verotoxin A method for detecting insertion of an NA fragment and a reagent for the detection. [Effect] Inactivation of the VT gene due to transfer of a foreign DNA fragment can be detected.
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