| abstract |
(57) [Problem] To provide a method for simply, quickly and accurately measuring free hemoglobin present in a liquid sample and a measurement kit. SOLUTION: A liquid sample containing free hemoglobin, a hemoglobin-haptoglobin complex and free haptoglobin is brought into contact with one end of a water-absorbing carrier containing an anti-haptoglobin antibody directly or via a blood cell separation membrane, The hemoglobin-haptoglobin complex and free haptoglobin in the liquid sample are captured, and then the free hemoglobin developed on the water-absorbing carrier without being captured and the first antibody contained in the water-absorbing carrier labeled with colored particles are contained. Free hemoglobin in the liquid sample can be visualized by binding the hemoglobin antibody and subsequently capturing the colored conjugate developed on the water-absorbing carrier with a second anti-hemoglobin antibody immobilized on the water-absorbing carrier. Detect and measure. |