http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H11187899-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d75d1a4caed60a9316608ed9c231546c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1997-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62cdb8062fcad16bf7ddea8c321fec4e |
| publicationDate | 1999-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H11187899-A |
| titleOfInvention | DNA sequencing method |
| abstract | (57) [Problem] To provide a method for determining a base sequence of DNA using RNA polymerase, which can determine the base sequence without using a labeled terminator. SOLUTION: A nucleic acid transcription product obtained by reacting ribonucleoside 5 triphosphates and 3 deoxyribonucleoside 5 triphosphate in the presence of an RNA polymerase and a DNA fragment containing a promoter sequence for the RNA polymerase is separated. And a method for determining the nucleotide sequence of a DNA for reading the sequence of a nucleic acid from the obtained separated fraction, wherein the initiator for the nucleic acid transcription reaction is oligoribonucleosides having no phosphate group at the 5 ′ end and 5 ′ At least one labeled nucleic acid transcription initiator consisting of oligoribonucleotides having a mono- or diphosphate group at the end is used. |
| priorityDate | 1997-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 212.