| abstract |
(57) [Summary] Phosphatase KU-8 which specifically dephosphorylates the 6-position bonded phosphate group among the 6- and 3-position bonded phosphate groups of a phosphorylated saccharide. And a method for producing a 3-position phosphorylated saccharide using the enzyme, the 3-position phosphorylated saccharide and its use. Also, a method for producing a 6-position phosphorylated saccharide, which is treated with a dilute acid, and the 6-position phosphorylated saccharide and its use. The novel phosphatase KU-8, which recognizes the binding position of the phosphate group of the phosphorylated sugar and performs specific dephosphorylation And a 3-position phosphorylated saccharide can be efficiently and easily obtained by using the present enzyme from a phosphorylated saccharide. Also, 6 A sugar that is phosphorylated only at the position is produced at low cost by acid treatment. Further, by using the present invention, the amount of phosphorus bound to the 6-position or 3-position of a glucose residue can be easily quantitatively analyzed. |