| abstract |
[PROBLEMS] To provide a method for easily producing a high-purity oligohyaluronic acid (salt) having a molecular weight of 10,000 or less at a low cost in a much simpler step than the conventional method. SOLUTION: A hyaluronic acid (salt) degrading enzyme is added to an aqueous solution of hyaluronic acid (salt) to decompose the hyaluronic acid (salt) at a temperature of 5 ° C to 50 ° C. The decomposing enzyme and the oligohyaluronic acid (salt) having a molecular weight of 10,000 or less are separated by using an ultrafiltration membrane, and sodium chloride or Add sodium acetate, Thereafter, the aqueous solution of the oligohyaluronic acid (salt) is crystallized by adding the aqueous solution of the oligohyaluronic acid (salt) to a poor solvent such as ethanol in an amount of 5 to 10 times the volume of the aqueous solution of the oligohyaluronic acid (salt), thereby obtaining a high-purity oligohyaluronic acid (salt). To manufacture. |