http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H11113599-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56ee5285e8e8edccb061046ff20da7a6 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1998-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_deae5c90adf053c2184a8e0437517908 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccec477641a760c136556fa17c69948f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d7a2b5ed7dc780ccf3ed552a976fcdf |
| publicationDate | 1999-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H11113599-A |
| titleOfInvention | Reduced cross contamination in nucleic acid amplification |
| abstract | (57) [Problem] To provide a simpler amplification method with reduced risk of contamination. A step of treating a sample with an enzyme under conditions in which an amplification product of a nucleic acid derived from another sample is degraded by an enzyme, a step of inactivating the enzyme, and a step of treating the sample under conditions in which the nucleic acid is amplified A method for amplifying a nucleic acid in a sample, which comprises a treating step (amplification is performed in the presence of a reagent for preventing the reactivation of the enzyme); a reagent for amplifying the nucleic acid, a nuclease, and a reagent for preventing the reactivation of the enzyme A method for preventing reactivation of uracil-N-glycosylase using N-lauroyl sarcosine; a method for detecting nucleic acid by the amplification method; and a method for detecting DNA polymerase at a low temperature using a surfactant. How to reduce unwanted side reactions. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE48909-E http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2002034905-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9284549-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10167500-B2 |
| priorityDate | 1997-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.