http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H11113573-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f97f0fa258a008bf056ad04b7f380dda |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 1997-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94b937a257975a29f2c303f4b13977db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ebc3b1f5819cc89b8e0cc66c68c7563 |
publicationDate | 1999-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H11113573-A |
titleOfInvention | Nucleic acid synthesis method |
abstract | (57) [Problem] To provide a novel method for efficiently amplifying DNA in a sample by suppressing the action of a PCR inhibitor. According to the present invention, in a nucleic acid synthesis method for amplifying a target gene in a sample, before performing nucleic acid synthesis, a gene amplification reaction solution to which the sample is added is heated to a temperature at which the thermostability of the thermostable enzyme is maintained. For example, by performing the treatment at 70 ° C. to 90 ° C. for 5 to 20 minutes, the action of the PCR inhibitor is suppressed. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017525383-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106715717-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106715717-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8669061-B2 |
priorityDate | 1997-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 151.