| abstract |
(57) Abstract. Specific ligands for E- and P-selectin were identified by combining wheat malt agglutinin agarose and E- or P-selectin rIg-agarose chromatography in vivo [ 3 H]. -Isolated from glucosamine labeled HL-60 cells. Total O- linked oligosaccharides on purification molecules liberated by alkaline hydrolysis of the NaBH 4 presence, specific exoglycosidase digestion in combination with ion - exchange, size - was analyzed by exclusion and filter paper chromatography. A variety of derived ligands and carbohydrates are claimed. |