Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-173845 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4725 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D41-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P43-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate |
1995-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate |
1998-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-H10506523-A |
titleOfInvention |
Method for purifying human recombinant decorin and method for detecting guanidium ion |
abstract |
(57) [Summary]nThe present invention relates to a method for the production of substantially pure human recombinant decorin. The method comprises contacting a decorin-containing cell culture medium with (1) a first strong anion exchange resin; then (2) a hydrophobic interaction chromatography resin; and finally (3) a second strong anion exchange resin. A distinct combination of three distinct steps. By using a combination of steps and specific reagents, especially a 2.4 to 3 molar GuHCl solution to elute decorin from a hydrophobic interaction column, the method of the present invention purifies human recombinant decorin. Provide a more convenient and reproducible method for The present invention also provides a method for detecting the presence of guanidium ions in a sample solution. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009535297-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014518508-A |
priorityDate |
1994-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |