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filingDate 1995-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1998-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H10504197-A
titleOfInvention Improved cleaning composition
abstract (57) Abstract A novel α-amylase variant derived from the DNA sequence of a naturally occurring or recombinant α-amylase has been disclosed. Generally, the mutant α-amylase encodes a naturally occurring or recombinant α-amylase to cause a substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of the precursor α-amylase. It is obtained by modifying the precursor DNA in vitro. Such a mutant α-amylase has altered oxidative stability and / or altered pH-effect properties and / or altered temperature stability compared to the precursor. Methods of using the mutant amylase and detergent and liquefied starch compositions comprising the mutant amylase are also disclosed. Particularly preferred is a mutant α-amylase modified in MET197 or MET15 from Bacillus licheniformis or at the residue of TRP138, or the corresponding residue of α-amylase from another microbial source (Bacillus, Aspergillus). It is a thing.
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