http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H10501410-A
Outgoing Links
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|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-34 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N63-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-325 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N37-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-325 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 1994-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1998-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H10501410-A |
| titleOfInvention | Identification, quantification and purification of insecticidal proteins from Bacillus thuringiensis |
| abstract | (57) Abstract: A method for identifying the protoxin of a protein expressed by the Bacillus thuringiensis gene is disclosed. According to this method, the daughter toxin, limited proteolysis with a protease in an aqueous suspension having a pH 9.5 greater than the protoxin-containing material, such as a parasporal crystal of Bacillus Churinji Enshisu First produced by exposure to The daughter toxin is then separated by high performance anion exchange liquid chromatography at a constant pH above 10 on an increasing gradient of salt, preferably sodium chloride. Gradient conditions that are specific to the column used are achieved by using a series of buffers with increasing concentrations of salt and introduced at a predetermined time and flow rate. The procedure provides a chromatogram showing clearly identifiable peaks of the toxin, thus permitting qualitative and quantitative characterization of the original mixture and isolation of individual toxins. Thereby, it provides a means to screen and test for new Bacillus thuringiensis, both single and duplicate genes, and to monitor the level of expression of known genes from known strains. The digestion and isolation conditions allow for the production of the toxin in a biologically active state. |
| priorityDate | 1990-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.