| abstract |
(57) Abstract: 3 '-(4'-) modified nucleosides and nucleotides of general formula (1) are described, wherein R' is hydrogen, mono-, di- or triphosphate, alkyl phosphonate, dialkyl phosphinate Or is a phosphoramidite; X is oxygen, nitrogen, carbon or sulfur; Y is oxygen, nitrogen or sulfur; Z is a hydrogen, hydroxyl, amino or thiol group and R is non-radioactive detectable A spacer group to which a group or effector molecule is attached; the base is a purine or pyrimidine base or deazapurine or a derivative thereof; n is 0 or 1. Methods for making these compounds and their use in nucleic acid analysis and drug manufacture are also described. In particular, 3'-amino-3'-deoxynucleotide fluorescein, in which the fluorescein group is linked to the sugar via a mono-, di- or tetraglycine unit, has been found to be advantageous in sequencing. |