| abstract |
(57) [Summary] (Modified) [Problem] An active form useful for analyzing the relationship between matrilysin, which is one of matrix metalloproteases, human matrix metalloproteinase 7 (human MMP-7), and various diseases Providing an accurate quantitative or measurement detection method for MMP-7. SOLUTION: A monoclonal antibody which specifically immunologically reacts only with human active MMP-7 by a cell fusion method using a region containing the amino acid sequence of YSLFP of MMP-7 or its vicinity as an immunogen can be obtained. Use this as a measurement reagent, In particular, a method and a reagent for measuring human activated MMP-7 by sandwich enzyme immunoassay are provided, which are active MMP-7 found in various cancer diseases including rectal cancer, prostate cancer, and the like, which are currently increasing. Can be detected, measured and quantified. These monoclonal antibodies are also useful as reagents for immunological staining of tissues and cells. |