| abstract |
PROBLEM TO BE SOLVED: To provide a novel enzyme having lactate oxidase activity which can be used for quantification of lactic acid, a gene encoding the enzyme, a method for producing the enzyme by genetic engineering technology, and Provision of its use. SOLUTION: Thermal stability of about 50 ° C. or less (pH 7.5, 1 0 min), pH stability about 4.0-9.0 (25 ° C, 16 hours), optimum temperature about 35 ° C, optimum pH about 6.5 Lactic acid oxidase derived from Lactococcus bacterium and a gene encoding the enzyme, a recombinant vector containing the gene, a transformant transformed with the vector, and culturing the transformant And a method for producing lactic acid oxidase and collecting the enzyme, and a reagent for measuring lactic acid. |