http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H10234389-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41686a979f961bce6a9855b4b409a5b8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1998-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95412f963ed86f235b08d847d89799f0 |
| publicationDate | 1998-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H10234389-A |
| titleOfInvention | Nucleic acid replication using single-stranded DNA binding proteins |
| abstract | (57) [Summary] [PROBLEMS] To significantly improve the amplification efficiency of a long target that cannot be efficiently amplified. SOLUTION: It has been confirmed that a single-stranded binding protein enhances the strand displacement replication efficiency of a long target. If added to an amplification reaction such as SDA, it is too long to efficiently amplify unless added. Significantly improves target amplification efficiency. The T4 gp32 protein is about 800-1,000b in length In the case of p, it has been shown that SDA results in about 10 6 to 10 8 fold amplification in 15 to 20 minutes. For targets of this length, there is essentially no detectable amplification in the absence of gp32. Targets about 400-500 bp in length can be amplified about 1 billion fold in the presence of gp32, and targets less than 100 bp in length can be amplified by conventional SDA reactions in the absence of SSB. It shows an amplification factor almost equivalent to. Other single-stranded DNA Binding proteins have also been shown to improve the replication and amplification efficiency of long targets. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011512821-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9422599-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008013010-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008048725-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7973131-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2005333920-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010263845-A |
| priorityDate | 1997-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 276.