abstract |
(57) [Summary] (Modifications) [Problem] To provide a gene encoding an esterase that produces an (R) -isomer by asymmetrically hydrolyzing an organic carboxylic acid ester of cyclopentenolones. SOLUTION: General formula 1 (Wherein, R 1 is hydrogen or a methyl group, R 2 is carbon atom 1 to 10 alkyl groups, alkenyl groups of 2 to 10 carbon atoms, alkynyl groups of 2 to 10 carbon atoms, 1 to 1 carbon atoms 4 haloalkyl groups, 5 to 9 hydroxyaliphatic hydrocarbon groups whose terminal hydroxy groups may be protected, or 5 to 9 carbon atom fatty acids whose terminal carboxyl groups may be protected An esterase gene that encodes an esterase that asymmetrically hydrolyzes an organic carboxylic acid ester of a cyclopentenolone represented by the following formula) to produce a cyclopentenolone represented by the general formula (1): Plasmids, transformants, and produced esterases related to the gene. |