| abstract |
(57) Abstract: Provided is a method for producing a nucleoside-5′-phosphate at low cost and efficiency by biochemically phosphorylating a nucleoside. The present invention relates to a mutant phosphatase having an increased affinity for acid phosphatase, particularly a nucleoside, and / or an improved temperature stability, and a microorganism into which a gene encoding a protein having the acid phosphatase activity is introduced. Nucleoside-5'-phosphate ester by reaction with a nucleoside and a phosphate donor selected from the group consisting of polyphosphoric acid (salt), phenylphosphoric acid (salt), carbamylphosphoric acid (salt), and acetylphosphoric acid (salt) under conditions Is generated and collected. |