| abstract |
(Solution) (Solution) Human ALT (alanine aminotransferas) e) the codon of 5 amino acids in the gene, ie, the fourth amino acid residue from the start codon methionine (Met) to the codon of serine (Ser), the fifth to the codon of threonine (Thr), and the seventh to asparagine Acid (As a modified human ALT in which the codon of p), the 39th position is replaced by a codon of glycine (Gly), and the 222nd position is replaced by a codon of alanine (Ala), and at the same time, restriction enzyme sites are added upstream and downstream of the gene. gene. [Effect] E. coli transformed with a recombinant plasmid obtained by linking a modified human ALT gene to a vector. By culturing E. coli, active human ALT having the same properties as the natural enzyme can be efficiently produced. |