http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H10179153-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad2cd796f8c4dcb425ddcc68707d3444 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-40 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate | 1996-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8565737c631a94f6ed31cc5be9866ae7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c13f3c1a5e1430db04fd3de7f19cd75c |
| publicationDate | 1998-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H10179153-A |
| titleOfInvention | 3α-hydroxysteroid dehydrogenase and method for producing the same |
| abstract | (57) [Summary] (Corrected) [PROBLEMS] To provide a novel 3α-hydroxysteroid dehydrogenase having a low Km value and a method for producing the same. A method for producing 3α-hydroxysteroid dehydrogenase having the following physicochemical properties and a bacterium belonging to the genus Pseudomonas. Action: 3α-hydroxysteroid + NAD + → 3-ketosteroid + NADH Substrate specificity: acts on cholic acid, deoxycholic acid, ursodeoxycholic acid, chenodeoxycholic acid, 6-ketolithocholic acid, lithocholic acid. Optimal temperature: 45 ° C, Optimum pH: pH 10-11, thermal stability: 40 ° C or less (p H 8.0, 10 minutes), pH stability: pH 6-9 (25 C, 16 hours), Km value for cholic acid: 0.015 mM, molecular weight: 25,000 (gel filtration method), 25.0 00 (SDS-PAGE) |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015506944-A |
| priorityDate | 1996-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 377.