http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H10174589-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80787665b837ed3eb503bbcd27c0043a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1997-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a3a06c3206ff0bb94506ab12baf6fe1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b40b55e7a09532a9dfb3fa663c6a29ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c266b77b2fb7af2b9a8e2089eaa86080 |
| publicationDate | 1998-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H10174589-A |
| titleOfInvention | DNA analysis method and reagent kit |
| abstract | (57) [Summary] [PROBLEMS] To provide a method for preparing a nested deletion library without using cloning and a method for determining a nucleotide sequence. SOLUTION: A DNA fragment (fragment-1) obtained by cleaving a sample DNA 151 using a restriction enzyme is amplified using 16 kinds of selective primer sets, and is amplified with λ exonuclease and Klenow fragment. DNA 152 (fragment-2) near one 5 'end of each fragment is obtained. A PCR reaction is performed using the fragment 2 and the sample DNA 151 to obtain a DNA fragment (third fragment) 158 containing a nucleotide sequence complementary to the fragment-2 and longer than this fragment. The third fragment has a common start and a different end depending on the restriction enzyme cleavage site. The nucleotide sequence is determined from the restriction enzyme cleavage site of each fragment, and the full-length nucleotide sequence of the sample DNA 151 is determined efficiently. |
| priorityDate | 1996-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 110.