Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_02ff914937eb6ceaee5ca69cc56cbfa7 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 |
filingDate |
1997-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cf275e9d93880ef75f2d2d35158b671 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe1dde0d3b6e26ae01ef718d0fb98d20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2801371ff4525b9a00fe6410bc7e53e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92748a7b284e4ddbf6238cab19fbc8bd |
publicationDate |
1998-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-H10155495-A |
titleOfInvention |
Method for producing phosphate-containing acidic sugar chain using yeast mannose-1-phosphotransferase gene |
abstract |
(57) Abstract: Yeast (Saccharomyces cerevisiae) yeast cells transformed with plasmid DNA into which a mannose-1-phosphotransferase gene has been introduced are cultured in a medium, and mannose-1 is isolated from the culture. -Obtaining a phosphotransferase and reacting it with a neutral core sugar chain in vivo or in vitro to obtain a mannose-1-phosphate-containing acidic sugar chain, thereby removing the mannose portion by acid treatment. A method for producing a phosphoric acid-containing acidic sugar chain, which is characterized in that: According to the present invention, it is possible to function as a marker for transporting a glycoprotein to lysosomes in mammalian cells such as humans by a genetic engineering technique using yeast, A useful acidic sugar chain identical to a useful human-derived phosphate-containing core sugar chain can be produced in a large amount and with high purity. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2002369692-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02103027-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4742191-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100888316-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-1298862-C |
priorityDate |
1997-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |