http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H09512900-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-967 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-962 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54313 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6408 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-75 |
| filingDate | 1995-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1997-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H09512900-A |
| titleOfInvention | Solid-phase assay for detection of ligand |
| abstract | (57) [Summary]nThe invention includes a method of detecting the presence or level of an analyte in a sample by detecting the formation of bound complexes on a solid phase. A preferred “competitive” method of the invention is a) mixing a sample with a second ligand capable of binding the analyte such that an analyte / second ligand complex is formed, and b) step ( The mixture produced in a) is contacted with a solid phase having a bound first ligand capable of binding a second ligand such that a first ligand / second ligand complex is formed, the contacting comprising: The analyte / second ligand complex formed in (a) is subjected to a sufficiently limited time under conditions that substantially prevent dissociation of the analyte / second ligand complex; and c) a detectable label is attached to the second ligand. Is bound before or after step (a) or (b) so that it is retained on the solid phase during formation of the first ligand / second ligand complex, and d) detecting this labeled moiety, The first phase on the solid phase allows the presence or level of the analyte in the sample to be determined. Comprising the step of detecting the formation of a ligand / second ligand complex. A preferred "sandwich-type" method of the present invention comprises: a) a solid phase having a sample, i) a first ligand bound to the analyte, and ii) a first ligand or a first ligand / analyte complex. A second ligand capable of binding to the body is contacted such that the first ligand / analyte / second ligand complex is formed on the solid phase, which contact is optional between the second ligand and the solid phase. For a sufficiently limited time under conditions that substantially block non-specific binding of b), b) a detectable label to the second ligand and the labeling moiety to the first ligand / analyte / second ligand complex. Bound either before or after formation of the first ligand / analyte / second ligand complex so that it is retained on the solid phase during formation of the body, c) detecting this label, Measuring the presence or level of the analyte in the sample. The invention also provides a method for single point calibration and quality assurance that can be used with the competitive and / or sandwich type assays described above. |
| priorityDate | 1994-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.