| abstract |
(57) [Summary] The present invention relates to a method for preparing clonogenic fibroblasts, which comprises excising and excising tissue from a donor, isolating individual cells from the tissue, and The resulting cell suspension is filtered, the cells contained in the cell suspension are washed, the cells are transformed into tissue culture without isolation of individual cells by mechanical trituration, then by collagenase alone. It is enzymatically treated and at least one gene is inserted into the fibroblasts by transfection, which gene is a biologically active protein, preferably a therapeutically active protein such as a growth factor, It encodes a hormone, enzyme, coagulation factor or coagulation inhibitor. |