| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96433 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1048
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-532
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07B61-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
1994-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
1997-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-H09502616-A |
| titleOfInvention |
Systematic evolution of ligands by exponential enrichment: Photoselection of nucleic acid ligands and solution SELEX |
| abstract |
(57) [Summary] A method for identifying a nucleic acid ligand for a target molecule using the SELEX operation, wherein the candidate nucleic acid contains a photoreactive group, and the ligand identified thereby are claimed. The high affinity nucleic acid-target molecule complex formed during this procedure is cross-linked by irradiation to facilitate separation from unbound nucleic acid. In other methods, the division of high-affinity nucleic acids and low-affinity nucleic acids is facilitated by a primer extension step, as shown in these methods, in which chain-terminating nucleotides, digestion-resistant nucleotides or cDNA products into an affinity matrix are used. Nucleotides that allow retention are variously incorporated into high or low affinity nucleic acids and the cDNA products are processed by amplification, enzymatic or chemical digestion or by contact with an affinity matrix. |
| priorityDate |
1993-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |