| abstract |
(57) [Summary]nThe present invention relates to a (1 → 3,1 → 4) -β-glucanase (glucanase EII endohydrolase) enzyme, the amino acid sequence of which imparts thermostability and / or pH stability with improved three-dimensional structure. It has been modified to supply the enzyme. The specific denaturation is based on a comparison between the three-dimensional structure of (1 → 3,1 → 4) -β-glucanase and the three-dimensional structure of (1 → 3) -β-glucanase. The (1 → 3,1 → 4) -β-glucanase gene has been modified by site-directed mutagenesis and the modified enzyme is expressed in E. coli. Modified sequences, DNA molecules encoding them, plasmids, expression vectors and transgenic plants are also disclosed. |