abstract |
(57) Abstract: PROBLEM TO BE SOLVED: To provide a screening method capable of selecting a protein binding to a specific target sequence, for example, an oncogene promoter sequence such as HRE-I sequence with high efficiency, and a plasmid used therefor, and HRE-. Providing a peptide that specifically binds to the I sequence. A plasmid preferably used for screening a peptide having a binding property to a target gene, comprising a base sequence encoding a test peptide whose binding degree to the target gene is evaluated, and the target gene. A promoter and a reporter gene operably linked to the promoter, the promoter controlling expression of the reporter gene according to the degree of binding of the peptide to the target gene contained therein. A plasmid comprising the target gene as defined above, and a peptide consisting of 7 residues having a specific sequence. |