| abstract |
(57) [Summary] [Structure] The surfactant, polyacyloxyethylene sorbitan ester, enables high density lipoprotein (HD Enzymes such as cholesterol esterase and cholesterol oxidase preferentially act on cholesterol in lipoprotein fractions other than L) to generate hydrogen peroxide. A colorless complex is formed in the presence of peroxidase to guide hydrogen peroxide out of the reaction system. Next, the surfactant alkyl polyoxyethylene ether suppresses the action of these enzymes on the cholesterol remaining in the lipoprotein fraction other than HDL, and also causes the enzyme to act on HDL cholesterol to generate hydrogen peroxide. . The quinone dye produced by hydrogen peroxide is colorimetrically measured to quantify HDL cholesterol. [Effect] The operation is simplified, and a large number of samples can be processed in a short time. Even a small amount of sample can be continuously measured by an automatic analyzer. It can contribute to the improvement of work efficiency in daily work of clinical examination. |