| abstract |
(57) [Abstract] [Problem] Pseudonocardia thermophila Pse udonocardiathermophila JC The amino acid sequence of nitrile hydratase derived from M3095 and the nucleotide sequence of the gene are provided. SOLUTION: Pseudonocardia thermophila Pseu The amino acid sequence of nitrile hydratase, which is derived from donocardia thermophila JCM3095 and is composed of two hetero subunits, and the nucleotide sequence of the gene are provided. In addition, a recombinant plasmid having the gene inserted therein is produced, and a nitrile compound is converted into a corresponding amide compound by using a transformed bacterial cell into which the recombinant plasmid has been introduced. [Effect] It becomes possible to efficiently convert a nitrile compound into a corresponding amide compound. |