http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H09252674-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dbe6c9c1497fd95f3aa892bf2e54a897 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
filingDate | 1996-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_220c17866976aaee528a65993a98c4e9 |
publicationDate | 1997-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H09252674-A |
titleOfInvention | Gene transfer method using Agrobacterium microorganism and method for producing transformed plant |
abstract | (57) Abstract: A tissue piece of a plant of the genus Brassica is pre-cultured in a tissue piece culture medium, and then immersed in an infection solution containing a microorganism of the genus Agrobacterium, and the tissue piece after the immersion is removed from the tissue. Cocultivation in a monoculture medium, then, by inducing callus from the tissue piece after coculture, by redifferentiating the obtained callus, in the method of producing a transformed plant of Brassica, in tissue tissue culture medium pH within a specific range, adding a feeder cell to the tissue piece culture medium, or immersion time in the infectious solution, temperature during co-cultivation, and co-cultivation period to three specific values, And a method for producing a transgenic plant of the genus Brassica. [Effect] To provide a method for efficiently introducing a gene into a plant of the genus Brassica to produce a transformed plant using a microorganism of the genus Agrobacterium. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010259378-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-100335618-C |
priorityDate | 1996-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.