| abstract |
(57) Abstract: An efficient method for purifying Tam-Horsfall glycoprotein (THG) and uromodulin and a method for distinguishing between them are provided. SOLUTION: Precipitated fraction produced by freezing and then thawing human urine is collected, or sodium or ammonium benzoate is dissolved in human urine and then the solution is acidified to produce benzoic acid, and the resulting precipitate fraction is collected. A method for purifying THG or uromodulin, which comprises collecting, purified products showing mutually different binding properties to human immunoglobulin classes and subclasses, and a method for distinguishing between them using the binding properties. [Effects] According to the present invention, THG and uromodulin can be purified with high efficiency by a simple operation, a purified product having unique properties can be obtained, and the properties can be utilized to distinguish between the two. it can. |