| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1fd34fea6801ea1d9286475b96be2d26 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-71
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-71 |
| filingDate |
1996-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_359f1b5529324cbca8311c2243095c06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11548909f99a9ae4ef6284d8e3dec42b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab7d91abe1ce1e3e00a5837e366b3629 |
| publicationDate |
1997-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-H09131184-A |
| titleOfInvention |
Vector, mammalian cell line, and screening method for antitumor agent |
| abstract |
(57) Abstract: A highly sensitive screening method for genetically engineered tumorigenic cell lines and antitumor agents. The present invention describes a novel method for measuring the amount of active tumor cells of a xenograft in an animal model. Genetically engineered cells that form tumors after transplantation synthesize secreted reporter proteins (eg, SEAP). SEAP activity in serum correlates with the number of active tumor cells in the animal and can be measured prior to palpable tumorigenesis. The application of this type of genetically altered cell line in subcutaneous and orthotopic transplant tumor models is also shown. For stable transfection, SE is under the control of constitutive or inducible promoter elements. A dicistronic eukaryotic expression vector is used that contains a gene encoding AP in association with a second gene. [Effect] The amount of active tumor cells in an animal model can be measured with high sensitivity by an enzyme test using serum or culture supernatant. |
| priorityDate |
1995-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |