http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H09107959-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9e1bf06f55de84b78abc9a057807f7eb |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-465 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
filingDate | 1995-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a59c450b47e6108c080c9657c5a00a38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e588fe753780ad42103bf8cfddc1716 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7ce00fb37541568d5a5bba6f2009f39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6af29c80ff1ec9f4a52a1abd98b0c5df |
publicationDate | 1997-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H09107959-A |
titleOfInvention | Malate dehydrogenase and method for producing the same |
abstract | (57) Abstract: A malate dehydrogenase having excellent storage stability and high specific activity is provided. A malate dehydrogenase having the following physicochemical properties. 1) Action Catalyze the following reactions: 2) Substrate specificity Km for oxaloacetate is 2.7x 10-5 M, 3) Optimum pH and stable pH range Optimum pH is 8-9 and stable at pH 7-11. 4) Molecular weight is 72,000 as measured by Sephadex G-100 gel chromatography. 5) Storage stability In 100 mM Tris-HCl buffer (pH 9), After being left for 20 days at room temperature (25 ° C.), it has a residual activity of 95% or more. 6) Thermostability 100 mM Tris- No inactivation is observed after treatment in a hydrochloric acid buffer solution (pH 9) at 50 ° C. for 15 minutes. 7) Specific activity: 1000 U / mg or more in the oxaloacetate reduction reaction. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006254795-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112592940-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112592940-A |
priorityDate | 1995-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 675.