abstract |
(57) [Summary]nA multi-purpose reagent system for rapid analysis of whole blood samples that enables at least 5 types of peripheral leukocytes, nucleated red blood cells and lymphocytes immunophenotypes to be determined with an automated hematology analyzer. The multipurpose reagent system rapidly lyses red blood cells while simultaneously fixing white blood cells and retaining lymphocyte surface antigens. The multipurpose reagent system comprises about 3 to 7 g / l of a non-quaternary ammonium salt, about 0.04 to about 0.10% by volume of an aliphatic aldehyde having 1 to 4 carbon atoms, and an aliphatic aldehyde-free An active non-phosphate buffer of about 10 mM to about 20 mM and sufficient water to bring the pH to about 5.5 to 7.5 and the osmolarity to about 160 to about 310 mOsm / l. Including |