abstract |
(57) [Summary]nThe present invention is a method of producing an expanded non-transformed cell culture, comprising: (1) preparing a partially purified, minced tissue; (2) enriching the resulting cells and tissue debris; (3) resuspending the enriched tissue cells and debris in a medium capable of supporting sustained cell division contained in a culture vessel; (4) incubating the cells; and (5 ) Providing the above method, which comprises the step of periodically subculturing the cells. The present invention relates to a clonal line of cells derived from the cell cultures described above; media and conditioned media designed for the culture of such cells, including pancreas, thyroid, parathyroid gland and parotid gland; and embedded in a substrate. For purifying pancreatic pseudotissues composed of aggregated (pseudoislets) or individual cells, for treating glycemic abnormalities in mammals, and for examining cytotoxic and autoimmune activity on pancreatic endocrine cells Further providing the use of cultured pancreatic cells |