http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H08505285-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6463 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6445 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01J4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate | 1993-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1996-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H08505285-A |
| titleOfInvention | Quantitative detection of macromolecules with fluorescent oligonucleotides |
| abstract | (57) [Summary]nDescribed is a method by which the association between an oligonucleotide labeled by binding a fluorophore and another macromolecule, such as a protein or a nucleic acid, can be quantified in solution accurately and with high sensitivity. In the practice of the present invention, the polarization of fluorescence of an exogenous fluorescent probe covalently attached to an oligonucleotide is measured. The change in fluorescence polarization is directly related to the degree of association of the labeled oligonucleotide with another macromolecule and can be used to quantify the association. Due to its high sensitivity and accuracy, the method of the present invention can be used to make reliable quantitative determinations of minute amounts of complexes formed between labeled oligonucleotides and proteins, nucleic acids or other macromolecules. The present invention also allows accurate estimation of important biochemical variables such as dissociation constants. Rapid and solution-based methods have wide application in biochemistry, gene cloning and molecular biology, and clinical diagnostics. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9960158-A1 |
| priorityDate | 1992-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 119.