http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H08505053-A
Outgoing Links
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|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00698 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B60-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00283 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00313 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00481 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00495 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0059 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00599 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00691 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J19-0046 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6862 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B60-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J19-00 |
| filingDate | 1993-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1996-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H08505053-A |
| titleOfInvention | Method and apparatus for enzymatic synthesis of oligonucleotides |
| abstract | (57) [Summary]nEnzymatic synthesis of oligonucleotides may be carried out in the following steps: (a) the primer and protected nucleotide are combined together in the presence of a chain extender, whereby the 3'-end of said primer is A primer protected nucleotide product containing bound protected nucleotides is formed; (b) removing the protecting group from the 3'-end of the primer protected nucleotide product; and (c) the primer of step (b). -Using the nucleotide product as a primer in step (a) of the next cycle, repeating the cycles of steps (a) and (b) for sufficient cycles to form the oligonucleotide product. Each cycle comprises deactivating the unreacted protected nucleotide substrate, i.e. converting any unreacted protected nucleotide into a substantially less active non-reactive form as a substrate for a chain extension enzyme. It may be done with or without. Each cycle may be carried out with or without removing the protecting group from the unreacted protected nucleotide. When the protected nucleotide used in one cycle should not be reused in the next cycle, the cycle deactivates the unreacted protected nucleotide, preferably removing the protecting group from the unreacted unprotected nucleotide. It is done by removing. If the protected nucleotides used in one cycle are to be reused in the next cycle, the cycle preferably does not remove the protecting groups from the unreacted unprotected nucleotides and preferably the unreacted protected nucleotides. It is carried out without deactivating the protected nucleotide. These cycles are preferably performed in a single vessel without intermediate purification of the oligonucleotide product. Removal of the protecting groups is preferably done enzymatically. Deactivation of unreacted protected nucleotides is preferably performed with an enzyme or combination of enzymes. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008545384-A |
| priorityDate | 1992-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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