| abstract |
(57) Summary An in vitro method for the production of chemically modified polyphenolic polymers (PPP). First, a stable, highly active extracellular tyrosinase is produced from a genetically transformed microorganism such as Streptomyces antibiotics. The tyrosinase is then incubated with the reaction substrate, eg 1-tyrosine, hydrolyzed protein or oligopeptide in combination with 1-tyrosine. The rate of oligopeptide / tyrosine pair synthesis and the concentration of tyrosinase can be used to modify the color, molecular weight and spectral absorption properties of the PPP produced. On the other hand, or in addition, an oxidizing agent, such as hydrogen peroxide or hypochlorite, is used to modify the color of PPP, despite the method used to produce PPP, and PPP is subsequently And can be fractionated using molecular weight cutoff ultrafiltration. Organic solvents can also be used in the process for producing PPP to produce PPP with various but reproducible physical properties. |