| abstract |
(57) [Summary] [Object] A gene encoding a protein that binds to inositol 1,4,5-triphosphates, a DNA fragment containing the gene, a transformant transformed with the fragment, and the transformation. Provided is a method for producing the above protein using the body. [Structure] A DNA probe was prepared based on a known partial amino acid sequence of a protein having a molecular weight of 130 Kd, and PCR was performed. Of the mRNA obtained from rat brain by the method of 5 Kbp The above mRNAs are separated, a cDNA library is prepared based on the mRNAs, and the clones producing the target protein are selected from the obtained clones. |