http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H08256797-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5128cfc84a0fb189fceb3cd240c44493 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| filingDate | 1995-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1629de564cd2ebbe1cf51f8a81e4d5e8 |
| publicationDate | 1996-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H08256797-A |
| titleOfInvention | Method for quantifying nucleic acid by enzyme and composition for quantification |
| abstract | (57) [Summary] [Structure] At least the following four reaction steps Step 1: A step of producing inorganic phosphorus in a nucleic acid in a sample by a phosphatase reaction, step 2: a deglycosylated purine derivative formed from the used nucleoside by subjecting the produced inorganic phosphorus to a nucleoside phosphorylase reaction in the presence of an excessive amount of nucleoside Alternatively, a deglycosylated pyrimidine derivative and a phosphorylated substrate are produced, and step 3: the produced phosphorylated substrate is made to produce inorganic phosphorus by the same phosphatase to cause the reaction of step 2 to carry out the reaction of step 2 and step 3 as a cycling reaction. Step 4: A method for quantifying a nucleic acid or a composition for quantifying, which comprises a step of enzymatically quantifying the amount of the deglycated purine derivative or the deglycated pyrimidine derivative produced in the whole process. [Effect] Compared with conventional nucleic acid quantification, it is possible to construct an enzymatic cycling reaction very easily and quantify nucleic acid with high sensitivity and reproducibility. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101835905-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009112195-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016152783-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014221072-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009057330-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011517571-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8435742-B2 |
| priorityDate | 1995-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 883.