abstract |
(57) Abstract: To discover and utilize an acarbose biosynthesis gene in Actinoplanes sp. Using a gene probe. The present invention relates to the acarbose biosynthesis gene from actinomycetes, in particular from Actinoplanes sp. SE 50/110 and its mutants, the gene acbA (dTD. P-glucose synthase), acbB (dT DP-glucose dehydratase) and acb C (code for cyclase, AroB, bacteria 3- (Partly the same as dehydroquinate synthase), or the known dTDP for the discovery of one or more acarbose biosynthetic genes from Actinoplanes sp. -Using a gene probe derived from the highly conserved protein region of the glucose dehydratase enzyme, The present invention relates to a method for isolating acarbose biosynthesis gene from actinomycetes and utilization of the acarbose biosynthesis gene. |