| abstract |
(57) [Summary] (Modified) [Objective] To provide a method for culturing and subculturing a novel segment bacterium. [Structure] The small intestinal epithelial cells of the animal were collected, subjected to chloroform treatment, and then aseptically administered to a sterile animal of the same species and kept aseptically for a certain period of time. Aseptic animals of the same kind, aseptically administered, aseptically raised for a certain period of time, further, if necessary, After this fecal collection and aseptic administration to a new animal were repeated several times, the animal became the only bacteria in the feces, A method of culturing and subculturing that confirms that it has a segment bacterium. [Effect] It is possible to provide a method for isolating segmented bacteria that are resident in the intestines of animals and have various physiological actions. |