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filingDate 1995-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1996-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H08208700-A
titleOfInvention Anti-human colorectal cancer mucus monoclonal antibody, method for producing the same, and colorectal cancer screening method using the antibody
abstract (57) [Summary] [Objective] To search for a marker different from conventional tumor markers, develop a monoclonal antibody that specifically recognizes the marker, and apply it to reliable colorectal cancer screening with a low false positive rate. With the goal. [Structure] An anti-human colon cancer mucus monoclonal antibody which belongs to the IgM class and recognizes a polysaccharide having a molecular weight of 250,000 to 500,000 in the colon mucus but does not recognize human hemoglobin. This antibody is used to immunize a mammal with colon mucus of a colorectal cancer patient whose human hemoglobin has been removed in advance as an immunizing antigen, B cell preparation step, cell fusion step, screening step, It can be obtained by carrying out a cloning step, a hybridoma culture step and an antibody separation and purification step. Colorectal cancer screening is performed by measuring the amount of the above-mentioned polysaccharide in human colon mucus collected from human colon or human colon mucus contained in human feces by an enzyme immunoassay using the above antibody. .
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