abstract |
(57) [Summary] [Structure] A novel β-galactoside-α2,6-sialyltransferase derived from a microorganism belonging to the genus Photobacterium and having the following physicochemical properties. (1) Action and specificity: Cytidine 1-phosphate can form sialic acid from sialic acid, a 6-position of a galactose residue in a glycoconjugate sugar chain or a free sugar chain, or a glycoconjugate of animal origin The monosaccharide is transferred to the 6-position of the monosaccharide having a hydroxyl group at the 6-position carbon. (2) Optimum pH: 5-6 (3) Optimum temperature: 30 ° C (4) Molecular weight: 64,000 ± 5,000 (by gel filtration) [Effect] Novel β-galactoside-α derived from microorganisms It is possible to provide 2,6-sialyltransferase, and it is possible to provide the present enzyme in a large amount and at low cost. |