| abstract |
(57) [Summary] [Object] To provide a method for mass-producing spheroids of desired size (desired number of cells) easily and inexpensively. [A composition] (A) a step of suspending a cell sheet or a spheroid precursor in the culture medium when the culture medium containing a gelling substance showing a sol-gel transition and having no adhesiveness to cells is in a sol state, A method for producing a spheroid, which comprises three steps: (B) immediately gelating the culture solution, and (C) culturing a cell sheet or a spheroid precursor in the gel. |