| abstract |
(57) [Summary] [PROBLEMS] To provide a purification of PACAP receptor protein or a salt thereof, a DNA containing a DNA encoding the protein, a method for producing the protein, the protein, and uses of the DNA. [Structure] The PACAP receptor protein was purified from bovine cerebrum. In addition, the DNA containing the DNA encoding the PACAP receptor protein was added to bovine, rat and human c It was isolated from a DNA library and its nucleotide sequence was determined. Furthermore, the amino acid sequence deduced from the base sequence was determined. Moreover, an antibody against the PACAP receptor protein or its partial peptide or a salt thereof was prepared. [Effect] The PACAP receptor protein of the present invention and the DNA encoding the protein can be used to obtain antibodies and antisera, construct a recombinant receptor protein expression system, Development of a receptor binding assay system using the same expression system, screening of drug candidate compounds, implementation of drug design based on comparison with structurally similar ligands and receptors, probes for gene diagnosis, PCR It can be used for preparing primers and the like. |