| abstract |
(57) [Summary] [Structure] α, α'-trehalose, 2,2'-dideoxy- Hydrolyzes α, α'-trehalose and 2-deoxy-α, α'-trehalose into their respective constituent sugars, but does not act on neotrehalose, lactose, maltose, cellobiose, sucrose, and has an optimum pH of pH5. ~ 6, optimum temperature is 65 ℃, has thermal stability up to 65 ℃, Molecular weight measured by gel filtration method is 400,000-500,00 And the molecular weight of the subunit by sodium dodecyl sulfate polyacrylamide gel electrophoresis is 180,000-25 A novel trehalase having a characteristic of being a glycoprotein, which has an isoelectric point of 2.7, measured by focal electrophoresis. [Effect] Since trehalose can be directly produced from glucose, the reaction is simple, there are no by-products, and it can be safely used as a food, and since trehalose is produced in high yield only with glucose, the cost is very low. Become. |